Evolutionary relationships among self-incompatibility RNases

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Evolutionary relationships among self-incompatibility RNases.

T2-type RNases are responsible for self-pollen recognition and rejection in three distantly related families of flowering plants-the Solanaceae, Scrophulariaceae, and Rosaceae. We used phylogenetic analyses of 67 T2-type RNases together with information on intron number and position to determine whether the use of RNases for self-incompatibility in these families is homologous or convergent. Al...

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Molecular modeling of S-RNases involved in almond self-incompatibility

Gametophytic self-incompatibility (GSI) is a mechanism in flowering plants, to prevent inbreeding and promote outcrossing. GSI is under the control of a specific locus, known as the S-locus, which contains at least two genes, the RNase and the SFB. Active S-RNases in the style are essential for rejection of haploid pollen, when the pollen S-allele matches one of two S-alleles of the diploid pis...

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Self-incompatibility RNases from three plant families: homology or convergence?

In the Rosaceae, Scrophulariaceae, and Solanaceae, the stylar product of the self-incompatibility (S-) locus is an RNase. Using protein sequence data from 34 RNase genes (three fungal RNases, seven angiosperm non-S RNases, 11 Rosaceae S-alleles, three Scrophulariaceae S-alleles, and ten Solanaceae S-alleles) we reconstructed the genealogy of angiosperm RNases using the neighbor joining method a...

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Evolutionary relationships among orbiviruses

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Identification of stylar RNases associated with gametophytic self-incompatibility in almond (Prunus dulcis).

Stylar proteins of 13 almond (Prunus dulcis) cultivars with known S-genotypes were surveyed by IEF and 2D-PAGE combined with immunoblot and N-terminal amino acid sequence analyses to identify S-RNases associated with gametophytic self-incompatibility (SI) in this plant species. RNase activities corresponding to Sa and Sb, two of the four S-alleles tested, were identified by IEF and RNase activi...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 2001

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.231386798